Confirmation of Plasmodium falciparum in vitro resistance to monodesethylamodiaquine and chloroquine in Dakar, Senegal, in 2015
Identifieur interne : 000103 ( France/Analysis ); précédent : 000102; suivant : 000104Confirmation of Plasmodium falciparum in vitro resistance to monodesethylamodiaquine and chloroquine in Dakar, Senegal, in 2015
Auteurs : Silman Diawara [Sénégal] ; Marylin Madamet [Sénégal, France] ; Mame Bou Kounta [Sénégal] ; Gora Lo [Sénégal] ; Khalifa Ababacar Wade [Sénégal] ; Aminata Nakoulima [Sénégal] ; Raymond Bercion [Sénégal] ; Remy Amalvict [Sénégal, France] ; Mamadou Wague Gueye [Sénégal] ; Bécaye Fall [Sénégal] ; Bakary Diatta [Sénégal] ; Bruno Pradines [Sénégal, France]Source :
- Malaria Journal [ 1475-2875 ] ; 2017-03.
Abstract
Background: In response to increasing resistance to anti-malarial drugs, Senegal adopted artemisinin-based combination therapy ( ACT) as the first-line treatment for uncomplicated malaria in 2006. However, resistance of Plasmodium falciparum parasites to artemisinin derivatives, characterized by delayed parasite clearance after treatment with ACT or artesunate monotherapy, has recently emerged and rapidly spread in Southeast Asia. After 10 years of stability with rates ranging from 5.6 to 11.8%, the prevalence of parasites with reduced susceptibility in vitro to monodesethylamodiaquine, the active metabolite of an ACT partner drug, increased to 30.6% in 2014 in Dakar. Additionally, after a decrease of the in vitro chloroquine resistance in Dakar in 2009-2011, the prevalence of parasites that showed in vitro chloroquine resistance increased again to approximately 50% in Dakar since 2013. The aim of this study was to follow the evolution of the susceptibility to ACT partners and other anti- malarial drugs in 2015 in Dakar. An in vitro test is the only method currently available to provide an early indication of resistance to ACT partners. Results: Thirty-two P. falciparum isolates collected in 2015 in Dakar were analysed using a standard ex vivo assay based on an HRP2 ELISA. The prevalence of P. falciparum parasites with reduced susceptibility in vitro to monodesethylamodiaquine, chloroquine, mefloquine, doxycycline and quinine was 28.1, 46.9, 45.2, 31.2 and 9.7%, respectively. None of the parasites were resistant to lumefantrine, piperaquine, pyronaridine, dihydroartemisinin and artesunate. These results confirm an increase in the reduced susceptibility to monodesethylamodiaquine observed in 2014 in Dakar and the chloroquine resistance observed in 2013. The in vitro resistance seems to be established in Dakar. Additionally, the prevalence of parasites with reduced susceptibility to doxycycline has increased two-fold compared to 2014. Conclusions: The establishment of a reduced susceptibility to monodesethylamodiaquine as well as chloroquine resistance, and the emergence of a reduced susceptibility to doxycycline are disturbing. The in vitro and in vivo surveillance of anti- malarial drugs must be implemented in Senegal.
Url:
DOI: 10.1186/s12936-017-1773-4
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Hal, to step Corpus: 000056
- to stream Hal, to step Curation: 000056
- to stream Hal, to step Checkpoint: 000061
- to stream Main, to step Merge: 000C75
- to stream Main, to step Curation: 000C75
- to stream Main, to step Exploration: 000C75
- to stream France, to step Extraction: 000103
Links to Exploration step
Hal:hal-01521245Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Confirmation of Plasmodium falciparum in vitro resistance to monodesethylamodiaquine and chloroquine in Dakar, Senegal, in 2015</title><author><name sortKey="Diawara, Silman" sort="Diawara, Silman" uniqKey="Diawara S" first="Silman" last="Diawara">Silman Diawara</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-338318" status="VALID"> <orgName>Hôpital Principal de Dakar</orgName> <desc> <address> <addrLine> 1, avenue Nelson Mandéla - B.P. 3006 - DAKAR (Sénégal)</addrLine> <country key="SN"></country> </address> <ref type="url">http://www.hopitalprincipal.sn/</ref> </desc> </hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Madamet, Marylin" sort="Madamet, Marylin" uniqKey="Madamet M" first="Marylin" last="Madamet">Marylin Madamet</name><affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-240602" status="OLD"> <orgName>Unité de Recherche sur les Maladies Infectieuses Tropicales Emergentes</orgName> <orgName type="acronym">URMITE</orgName> <date type="end">3107-12-31</date> <desc> <address> <addrLine>Campus International de Hann - BP 1386 - CP 18524 Dakar</addrLine> <country key="SN"></country> </address> </desc> <listRelation> <relation active="#struct-199392" type="direct"></relation> <relation name="UMR_D198" active="#struct-67872" type="indirect"></relation> <relation name="UM63" active="#struct-198056" type="indirect"></relation> <relation name="U1095" active="#struct-303623" type="indirect"></relation> <relation active="#struct-303759" type="indirect"></relation> <relation active="#struct-302108" type="indirect"></relation> <relation name="UMR7278" active="#struct-441569" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-199392" type="direct"><org type="laboratory" xml:id="struct-199392" status="OLD"> <idno type="IdRef">123037891</idno> <idno type="RNSR">200817431S</idno> <orgName>Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes</orgName> <orgName type="acronym">URMITE</orgName> <date type="start">2008-01-01</date> <date type="end">2017-12-31</date> <desc> <address> <addrLine>27 Bd Jean Moulin 13385 MARSEILLE Cedex 5</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.mediterranee-infection.com/</ref> </desc> <listRelation> <relation name="UMR_D198" active="#struct-67872" type="direct"></relation> <relation name="UM63" active="#struct-198056" type="direct"></relation> <relation name="U1095" active="#struct-303623" type="direct"></relation> <relation active="#struct-303759" type="direct"></relation> <relation active="#struct-302108" type="indirect"></relation> <relation name="UMR7278" active="#struct-441569" type="direct"></relation> </listRelation> </org></tutelle><tutelle name="UMR_D198" active="#struct-67872" type="indirect"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName> <orgName type="acronym">IRD</orgName> <desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.ird.fr/</ref> </desc> </org></tutelle><tutelle name="UM63" active="#struct-198056" type="indirect"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno> <idno type="ISNI">0000 0001 2176 4817</idno> <orgName>Aix Marseille Université</orgName> <orgName type="acronym">AMU</orgName> <date type="start">2012-01-01</date> <desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-amu.fr/</ref> </desc> </org></tutelle><tutelle name="U1095" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno> <orgName>Institut National de la Santé et de la Recherche Médicale</orgName> <orgName type="acronym">INSERM</orgName> <desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.inserm.fr</ref> </desc> </org></tutelle><tutelle active="#struct-303759" type="indirect"><org type="regrouplaboratory" xml:id="struct-303759" status="OLD"> <orgName>IFR48</orgName> <date type="start">1996-01-01</date> <date type="end">2011-12-31</date> <desc> <address> <country key="FR"></country> </address> </desc> <listRelation> <relation active="#struct-302108" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-302108" type="indirect"><org type="institution" xml:id="struct-302108" status="VALID"> <orgName>INSB</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle name="UMR7278" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Sénégal</country><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName><country>France</country><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Kounta, Mame Bou" sort="Kounta, Mame Bou" uniqKey="Kounta M" first="Mame Bou" last="Kounta">Mame Bou Kounta</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-338318" status="VALID"> <orgName>Hôpital Principal de Dakar</orgName> <desc> <address> <addrLine> 1, avenue Nelson Mandéla - B.P. 3006 - DAKAR (Sénégal)</addrLine> <country key="SN"></country> </address> <ref type="url">http://www.hopitalprincipal.sn/</ref> </desc> </hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Lo, Gora" sort="Lo, Gora" uniqKey="Lo G" first="Gora" last="Lo">Gora Lo</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-301776" status="VALID"> <orgName>Université Cheikh Anta Diop [Dakar, Sénégal]</orgName> <orgName type="acronym">UCAD</orgName> <desc> <address> <addrLine>Dakar</addrLine> <country key="SN"></country> </address> <ref type="url">https://www.ucad.sn/</ref> </desc> </hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Wade, Khalifa Ababacar" sort="Wade, Khalifa Ababacar" uniqKey="Wade K" first="Khalifa Ababacar" last="Wade">Khalifa Ababacar Wade</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-338318" status="VALID"> <orgName>Hôpital Principal de Dakar</orgName> <desc> <address> <addrLine> 1, avenue Nelson Mandéla - B.P. 3006 - DAKAR (Sénégal)</addrLine> <country key="SN"></country> </address> <ref type="url">http://www.hopitalprincipal.sn/</ref> </desc> </hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Nakoulima, Aminata" sort="Nakoulima, Aminata" uniqKey="Nakoulima A" first="Aminata" last="Nakoulima">Aminata Nakoulima</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-338318" status="VALID"> <orgName>Hôpital Principal de Dakar</orgName> <desc> <address> <addrLine> 1, avenue Nelson Mandéla - B.P. 3006 - DAKAR (Sénégal)</addrLine> <country key="SN"></country> </address> <ref type="url">http://www.hopitalprincipal.sn/</ref> </desc> </hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Bercion, Raymond" sort="Bercion, Raymond" uniqKey="Bercion R" first="Raymond" last="Bercion">Raymond Bercion</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-42095" status="VALID"> <orgName>Institut Pasteur de Dakar</orgName> <desc> <address> <addrLine>B.P. 220 Dakar</addrLine> <country key="SN"></country> </address> <ref type="url">http://www.pasteur.sn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-301247" type="direct"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Amalvict, Remy" sort="Amalvict, Remy" uniqKey="Amalvict R" first="Remy" last="Amalvict">Remy Amalvict</name><affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-240602" status="OLD"> <orgName>Unité de Recherche sur les Maladies Infectieuses Tropicales Emergentes</orgName> <orgName type="acronym">URMITE</orgName> <date type="end">3107-12-31</date> <desc> <address> <addrLine>Campus International de Hann - BP 1386 - CP 18524 Dakar</addrLine> <country key="SN"></country> </address> </desc> <listRelation> <relation active="#struct-199392" type="direct"></relation> <relation name="UMR_D198" active="#struct-67872" type="indirect"></relation> <relation name="UM63" active="#struct-198056" type="indirect"></relation> <relation name="U1095" active="#struct-303623" type="indirect"></relation> <relation active="#struct-303759" type="indirect"></relation> <relation active="#struct-302108" type="indirect"></relation> <relation name="UMR7278" active="#struct-441569" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-199392" type="direct"><org type="laboratory" xml:id="struct-199392" status="OLD"> <idno type="IdRef">123037891</idno> <idno type="RNSR">200817431S</idno> <orgName>Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes</orgName> <orgName type="acronym">URMITE</orgName> <date type="start">2008-01-01</date> <date type="end">2017-12-31</date> <desc> <address> <addrLine>27 Bd Jean Moulin 13385 MARSEILLE Cedex 5</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.mediterranee-infection.com/</ref> </desc> <listRelation> <relation name="UMR_D198" active="#struct-67872" type="direct"></relation> <relation name="UM63" active="#struct-198056" type="direct"></relation> <relation name="U1095" active="#struct-303623" type="direct"></relation> <relation active="#struct-303759" type="direct"></relation> <relation active="#struct-302108" type="indirect"></relation> <relation name="UMR7278" active="#struct-441569" type="direct"></relation> </listRelation> </org></tutelle><tutelle name="UMR_D198" active="#struct-67872" type="indirect"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName> <orgName type="acronym">IRD</orgName> <desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.ird.fr/</ref> </desc> </org></tutelle><tutelle name="UM63" active="#struct-198056" type="indirect"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno> <idno type="ISNI">0000 0001 2176 4817</idno> <orgName>Aix Marseille Université</orgName> <orgName type="acronym">AMU</orgName> <date type="start">2012-01-01</date> <desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-amu.fr/</ref> </desc> </org></tutelle><tutelle name="U1095" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno> <orgName>Institut National de la Santé et de la Recherche Médicale</orgName> <orgName type="acronym">INSERM</orgName> <desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.inserm.fr</ref> </desc> </org></tutelle><tutelle active="#struct-303759" type="indirect"><org type="regrouplaboratory" xml:id="struct-303759" status="OLD"> <orgName>IFR48</orgName> <date type="start">1996-01-01</date> <date type="end">2011-12-31</date> <desc> <address> <country key="FR"></country> </address> </desc> <listRelation> <relation active="#struct-302108" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-302108" type="indirect"><org type="institution" xml:id="struct-302108" status="VALID"> <orgName>INSB</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle name="UMR7278" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Sénégal</country><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName><country>France</country><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Gueye, Mamadou Wague" sort="Gueye, Mamadou Wague" uniqKey="Gueye M" first="Mamadou Wague" last="Gueye">Mamadou Wague Gueye</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-338318" status="VALID"> <orgName>Hôpital Principal de Dakar</orgName> <desc> <address> <addrLine> 1, avenue Nelson Mandéla - B.P. 3006 - DAKAR (Sénégal)</addrLine> <country key="SN"></country> </address> <ref type="url">http://www.hopitalprincipal.sn/</ref> </desc> </hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Fall, Becaye" sort="Fall, Becaye" uniqKey="Fall B" first="Bécaye" last="Fall">Bécaye Fall</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-338318" status="VALID"> <orgName>Hôpital Principal de Dakar</orgName> <desc> <address> <addrLine> 1, avenue Nelson Mandéla - B.P. 3006 - DAKAR (Sénégal)</addrLine> <country key="SN"></country> </address> <ref type="url">http://www.hopitalprincipal.sn/</ref> </desc> </hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Diatta, Bakary" sort="Diatta, Bakary" uniqKey="Diatta B" first="Bakary" last="Diatta">Bakary Diatta</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-338318" status="VALID"> <orgName>Hôpital Principal de Dakar</orgName> <desc> <address> <addrLine> 1, avenue Nelson Mandéla - B.P. 3006 - DAKAR (Sénégal)</addrLine> <country key="SN"></country> </address> <ref type="url">http://www.hopitalprincipal.sn/</ref> </desc> </hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Pradines, Bruno" sort="Pradines, Bruno" uniqKey="Pradines B" first="Bruno" last="Pradines">Bruno Pradines</name><affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-240602" status="OLD"> <orgName>Unité de Recherche sur les Maladies Infectieuses Tropicales Emergentes</orgName> <orgName type="acronym">URMITE</orgName> <date type="end">3107-12-31</date> <desc> <address> <addrLine>Campus International de Hann - BP 1386 - CP 18524 Dakar</addrLine> <country key="SN"></country> </address> </desc> <listRelation> <relation active="#struct-199392" type="direct"></relation> <relation name="UMR_D198" active="#struct-67872" type="indirect"></relation> <relation name="UM63" active="#struct-198056" type="indirect"></relation> <relation name="U1095" active="#struct-303623" type="indirect"></relation> <relation active="#struct-303759" type="indirect"></relation> <relation active="#struct-302108" type="indirect"></relation> <relation name="UMR7278" active="#struct-441569" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-199392" type="direct"><org type="laboratory" xml:id="struct-199392" status="OLD"> <idno type="IdRef">123037891</idno> <idno type="RNSR">200817431S</idno> <orgName>Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes</orgName> <orgName type="acronym">URMITE</orgName> <date type="start">2008-01-01</date> <date type="end">2017-12-31</date> <desc> <address> <addrLine>27 Bd Jean Moulin 13385 MARSEILLE Cedex 5</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.mediterranee-infection.com/</ref> </desc> <listRelation> <relation name="UMR_D198" active="#struct-67872" type="direct"></relation> <relation name="UM63" active="#struct-198056" type="direct"></relation> <relation name="U1095" active="#struct-303623" type="direct"></relation> <relation active="#struct-303759" type="direct"></relation> <relation active="#struct-302108" type="indirect"></relation> <relation name="UMR7278" active="#struct-441569" type="direct"></relation> </listRelation> </org></tutelle><tutelle name="UMR_D198" active="#struct-67872" type="indirect"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName> <orgName type="acronym">IRD</orgName> <desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.ird.fr/</ref> </desc> </org></tutelle><tutelle name="UM63" active="#struct-198056" type="indirect"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno> <idno type="ISNI">0000 0001 2176 4817</idno> <orgName>Aix Marseille Université</orgName> <orgName type="acronym">AMU</orgName> <date type="start">2012-01-01</date> <desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-amu.fr/</ref> </desc> </org></tutelle><tutelle name="U1095" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno> <orgName>Institut National de la Santé et de la Recherche Médicale</orgName> <orgName type="acronym">INSERM</orgName> <desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.inserm.fr</ref> </desc> </org></tutelle><tutelle active="#struct-303759" type="indirect"><org type="regrouplaboratory" xml:id="struct-303759" status="OLD"> <orgName>IFR48</orgName> <date type="start">1996-01-01</date> <date type="end">2011-12-31</date> <desc> <address> <country key="FR"></country> </address> </desc> <listRelation> <relation active="#struct-302108" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-302108" type="indirect"><org type="institution" xml:id="struct-302108" status="VALID"> <orgName>INSB</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle name="UMR7278" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Sénégal</country><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName><country>France</country><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">HAL</idno><idno type="RBID">Hal:hal-01521245</idno><idno type="halId">hal-01521245</idno><idno type="halUri">https://hal.archives-ouvertes.fr/hal-01521245</idno><idno type="url">https://hal.archives-ouvertes.fr/hal-01521245</idno><idno type="doi">10.1186/s12936-017-1773-4</idno><date when="2017-03">2017-03</date><idno type="wicri:Area/Hal/Corpus">000056</idno><idno type="wicri:Area/Hal/Curation">000056</idno><idno type="wicri:Area/Hal/Checkpoint">000061</idno><idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000061</idno><idno type="wicri:doubleKey">1475-2875:2017:Diawara S:confirmation:of:plasmodium</idno><idno type="wicri:Area/Main/Merge">000C75</idno><idno type="wicri:Area/Main/Curation">000C75</idno><idno type="wicri:Area/Main/Exploration">000C75</idno><idno type="wicri:Area/France/Extraction">000103</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Confirmation of Plasmodium falciparum in vitro resistance to monodesethylamodiaquine and chloroquine in Dakar, Senegal, in 2015</title><author><name sortKey="Diawara, Silman" sort="Diawara, Silman" uniqKey="Diawara S" first="Silman" last="Diawara">Silman Diawara</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-338318" status="VALID"> <orgName>Hôpital Principal de Dakar</orgName> <desc> <address> <addrLine> 1, avenue Nelson Mandéla - B.P. 3006 - DAKAR (Sénégal)</addrLine> <country key="SN"></country> </address> <ref type="url">http://www.hopitalprincipal.sn/</ref> </desc> </hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Madamet, Marylin" sort="Madamet, Marylin" uniqKey="Madamet M" first="Marylin" last="Madamet">Marylin Madamet</name><affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-240602" status="OLD"> <orgName>Unité de Recherche sur les Maladies Infectieuses Tropicales Emergentes</orgName> <orgName type="acronym">URMITE</orgName> <date type="end">3107-12-31</date> <desc> <address> <addrLine>Campus International de Hann - BP 1386 - CP 18524 Dakar</addrLine> <country key="SN"></country> </address> </desc> <listRelation> <relation active="#struct-199392" type="direct"></relation> <relation name="UMR_D198" active="#struct-67872" type="indirect"></relation> <relation name="UM63" active="#struct-198056" type="indirect"></relation> <relation name="U1095" active="#struct-303623" type="indirect"></relation> <relation active="#struct-303759" type="indirect"></relation> <relation active="#struct-302108" type="indirect"></relation> <relation name="UMR7278" active="#struct-441569" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-199392" type="direct"><org type="laboratory" xml:id="struct-199392" status="OLD"> <idno type="IdRef">123037891</idno> <idno type="RNSR">200817431S</idno> <orgName>Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes</orgName> <orgName type="acronym">URMITE</orgName> <date type="start">2008-01-01</date> <date type="end">2017-12-31</date> <desc> <address> <addrLine>27 Bd Jean Moulin 13385 MARSEILLE Cedex 5</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.mediterranee-infection.com/</ref> </desc> <listRelation> <relation name="UMR_D198" active="#struct-67872" type="direct"></relation> <relation name="UM63" active="#struct-198056" type="direct"></relation> <relation name="U1095" active="#struct-303623" type="direct"></relation> <relation active="#struct-303759" type="direct"></relation> <relation active="#struct-302108" type="indirect"></relation> <relation name="UMR7278" active="#struct-441569" type="direct"></relation> </listRelation> </org></tutelle><tutelle name="UMR_D198" active="#struct-67872" type="indirect"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName> <orgName type="acronym">IRD</orgName> <desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.ird.fr/</ref> </desc> </org></tutelle><tutelle name="UM63" active="#struct-198056" type="indirect"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno> <idno type="ISNI">0000 0001 2176 4817</idno> <orgName>Aix Marseille Université</orgName> <orgName type="acronym">AMU</orgName> <date type="start">2012-01-01</date> <desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-amu.fr/</ref> </desc> </org></tutelle><tutelle name="U1095" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno> <orgName>Institut National de la Santé et de la Recherche Médicale</orgName> <orgName type="acronym">INSERM</orgName> <desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.inserm.fr</ref> </desc> </org></tutelle><tutelle active="#struct-303759" type="indirect"><org type="regrouplaboratory" xml:id="struct-303759" status="OLD"> <orgName>IFR48</orgName> <date type="start">1996-01-01</date> <date type="end">2011-12-31</date> <desc> <address> <country key="FR"></country> </address> </desc> <listRelation> <relation active="#struct-302108" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-302108" type="indirect"><org type="institution" xml:id="struct-302108" status="VALID"> <orgName>INSB</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle name="UMR7278" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Sénégal</country><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName><country>France</country><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Kounta, Mame Bou" sort="Kounta, Mame Bou" uniqKey="Kounta M" first="Mame Bou" last="Kounta">Mame Bou Kounta</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-338318" status="VALID"> <orgName>Hôpital Principal de Dakar</orgName> <desc> <address> <addrLine> 1, avenue Nelson Mandéla - B.P. 3006 - DAKAR (Sénégal)</addrLine> <country key="SN"></country> </address> <ref type="url">http://www.hopitalprincipal.sn/</ref> </desc> </hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Lo, Gora" sort="Lo, Gora" uniqKey="Lo G" first="Gora" last="Lo">Gora Lo</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-301776" status="VALID"> <orgName>Université Cheikh Anta Diop [Dakar, Sénégal]</orgName> <orgName type="acronym">UCAD</orgName> <desc> <address> <addrLine>Dakar</addrLine> <country key="SN"></country> </address> <ref type="url">https://www.ucad.sn/</ref> </desc> </hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Wade, Khalifa Ababacar" sort="Wade, Khalifa Ababacar" uniqKey="Wade K" first="Khalifa Ababacar" last="Wade">Khalifa Ababacar Wade</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-338318" status="VALID"> <orgName>Hôpital Principal de Dakar</orgName> <desc> <address> <addrLine> 1, avenue Nelson Mandéla - B.P. 3006 - DAKAR (Sénégal)</addrLine> <country key="SN"></country> </address> <ref type="url">http://www.hopitalprincipal.sn/</ref> </desc> </hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Nakoulima, Aminata" sort="Nakoulima, Aminata" uniqKey="Nakoulima A" first="Aminata" last="Nakoulima">Aminata Nakoulima</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-338318" status="VALID"> <orgName>Hôpital Principal de Dakar</orgName> <desc> <address> <addrLine> 1, avenue Nelson Mandéla - B.P. 3006 - DAKAR (Sénégal)</addrLine> <country key="SN"></country> </address> <ref type="url">http://www.hopitalprincipal.sn/</ref> </desc> </hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Bercion, Raymond" sort="Bercion, Raymond" uniqKey="Bercion R" first="Raymond" last="Bercion">Raymond Bercion</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-42095" status="VALID"> <orgName>Institut Pasteur de Dakar</orgName> <desc> <address> <addrLine>B.P. 220 Dakar</addrLine> <country key="SN"></country> </address> <ref type="url">http://www.pasteur.sn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-301247" type="direct"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Amalvict, Remy" sort="Amalvict, Remy" uniqKey="Amalvict R" first="Remy" last="Amalvict">Remy Amalvict</name><affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-240602" status="OLD"> <orgName>Unité de Recherche sur les Maladies Infectieuses Tropicales Emergentes</orgName> <orgName type="acronym">URMITE</orgName> <date type="end">3107-12-31</date> <desc> <address> <addrLine>Campus International de Hann - BP 1386 - CP 18524 Dakar</addrLine> <country key="SN"></country> </address> </desc> <listRelation> <relation active="#struct-199392" type="direct"></relation> <relation name="UMR_D198" active="#struct-67872" type="indirect"></relation> <relation name="UM63" active="#struct-198056" type="indirect"></relation> <relation name="U1095" active="#struct-303623" type="indirect"></relation> <relation active="#struct-303759" type="indirect"></relation> <relation active="#struct-302108" type="indirect"></relation> <relation name="UMR7278" active="#struct-441569" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-199392" type="direct"><org type="laboratory" xml:id="struct-199392" status="OLD"> <idno type="IdRef">123037891</idno> <idno type="RNSR">200817431S</idno> <orgName>Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes</orgName> <orgName type="acronym">URMITE</orgName> <date type="start">2008-01-01</date> <date type="end">2017-12-31</date> <desc> <address> <addrLine>27 Bd Jean Moulin 13385 MARSEILLE Cedex 5</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.mediterranee-infection.com/</ref> </desc> <listRelation> <relation name="UMR_D198" active="#struct-67872" type="direct"></relation> <relation name="UM63" active="#struct-198056" type="direct"></relation> <relation name="U1095" active="#struct-303623" type="direct"></relation> <relation active="#struct-303759" type="direct"></relation> <relation active="#struct-302108" type="indirect"></relation> <relation name="UMR7278" active="#struct-441569" type="direct"></relation> </listRelation> </org></tutelle><tutelle name="UMR_D198" active="#struct-67872" type="indirect"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName> <orgName type="acronym">IRD</orgName> <desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.ird.fr/</ref> </desc> </org></tutelle><tutelle name="UM63" active="#struct-198056" type="indirect"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno> <idno type="ISNI">0000 0001 2176 4817</idno> <orgName>Aix Marseille Université</orgName> <orgName type="acronym">AMU</orgName> <date type="start">2012-01-01</date> <desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-amu.fr/</ref> </desc> </org></tutelle><tutelle name="U1095" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno> <orgName>Institut National de la Santé et de la Recherche Médicale</orgName> <orgName type="acronym">INSERM</orgName> <desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.inserm.fr</ref> </desc> </org></tutelle><tutelle active="#struct-303759" type="indirect"><org type="regrouplaboratory" xml:id="struct-303759" status="OLD"> <orgName>IFR48</orgName> <date type="start">1996-01-01</date> <date type="end">2011-12-31</date> <desc> <address> <country key="FR"></country> </address> </desc> <listRelation> <relation active="#struct-302108" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-302108" type="indirect"><org type="institution" xml:id="struct-302108" status="VALID"> <orgName>INSB</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle name="UMR7278" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Sénégal</country><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName><country>France</country><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Gueye, Mamadou Wague" sort="Gueye, Mamadou Wague" uniqKey="Gueye M" first="Mamadou Wague" last="Gueye">Mamadou Wague Gueye</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-338318" status="VALID"> <orgName>Hôpital Principal de Dakar</orgName> <desc> <address> <addrLine> 1, avenue Nelson Mandéla - B.P. 3006 - DAKAR (Sénégal)</addrLine> <country key="SN"></country> </address> <ref type="url">http://www.hopitalprincipal.sn/</ref> </desc> </hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Fall, Becaye" sort="Fall, Becaye" uniqKey="Fall B" first="Bécaye" last="Fall">Bécaye Fall</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-338318" status="VALID"> <orgName>Hôpital Principal de Dakar</orgName> <desc> <address> <addrLine> 1, avenue Nelson Mandéla - B.P. 3006 - DAKAR (Sénégal)</addrLine> <country key="SN"></country> </address> <ref type="url">http://www.hopitalprincipal.sn/</ref> </desc> </hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Diatta, Bakary" sort="Diatta, Bakary" uniqKey="Diatta B" first="Bakary" last="Diatta">Bakary Diatta</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-338318" status="VALID"> <orgName>Hôpital Principal de Dakar</orgName> <desc> <address> <addrLine> 1, avenue Nelson Mandéla - B.P. 3006 - DAKAR (Sénégal)</addrLine> <country key="SN"></country> </address> <ref type="url">http://www.hopitalprincipal.sn/</ref> </desc> </hal:affiliation><country>Sénégal</country></affiliation></author><author><name sortKey="Pradines, Bruno" sort="Pradines, Bruno" uniqKey="Pradines B" first="Bruno" last="Pradines">Bruno Pradines</name><affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-240602" status="OLD"> <orgName>Unité de Recherche sur les Maladies Infectieuses Tropicales Emergentes</orgName> <orgName type="acronym">URMITE</orgName> <date type="end">3107-12-31</date> <desc> <address> <addrLine>Campus International de Hann - BP 1386 - CP 18524 Dakar</addrLine> <country key="SN"></country> </address> </desc> <listRelation> <relation active="#struct-199392" type="direct"></relation> <relation name="UMR_D198" active="#struct-67872" type="indirect"></relation> <relation name="UM63" active="#struct-198056" type="indirect"></relation> <relation name="U1095" active="#struct-303623" type="indirect"></relation> <relation active="#struct-303759" type="indirect"></relation> <relation active="#struct-302108" type="indirect"></relation> <relation name="UMR7278" active="#struct-441569" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-199392" type="direct"><org type="laboratory" xml:id="struct-199392" status="OLD"> <idno type="IdRef">123037891</idno> <idno type="RNSR">200817431S</idno> <orgName>Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes</orgName> <orgName type="acronym">URMITE</orgName> <date type="start">2008-01-01</date> <date type="end">2017-12-31</date> <desc> <address> <addrLine>27 Bd Jean Moulin 13385 MARSEILLE Cedex 5</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.mediterranee-infection.com/</ref> </desc> <listRelation> <relation name="UMR_D198" active="#struct-67872" type="direct"></relation> <relation name="UM63" active="#struct-198056" type="direct"></relation> <relation name="U1095" active="#struct-303623" type="direct"></relation> <relation active="#struct-303759" type="direct"></relation> <relation active="#struct-302108" type="indirect"></relation> <relation name="UMR7278" active="#struct-441569" type="direct"></relation> </listRelation> </org></tutelle><tutelle name="UMR_D198" active="#struct-67872" type="indirect"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName> <orgName type="acronym">IRD</orgName> <desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.ird.fr/</ref> </desc> </org></tutelle><tutelle name="UM63" active="#struct-198056" type="indirect"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno> <idno type="ISNI">0000 0001 2176 4817</idno> <orgName>Aix Marseille Université</orgName> <orgName type="acronym">AMU</orgName> <date type="start">2012-01-01</date> <desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-amu.fr/</ref> </desc> </org></tutelle><tutelle name="U1095" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno> <orgName>Institut National de la Santé et de la Recherche Médicale</orgName> <orgName type="acronym">INSERM</orgName> <desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.inserm.fr</ref> </desc> </org></tutelle><tutelle active="#struct-303759" type="indirect"><org type="regrouplaboratory" xml:id="struct-303759" status="OLD"> <orgName>IFR48</orgName> <date type="start">1996-01-01</date> <date type="end">2011-12-31</date> <desc> <address> <country key="FR"></country> </address> </desc> <listRelation> <relation active="#struct-302108" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-302108" type="indirect"><org type="institution" xml:id="struct-302108" status="VALID"> <orgName>INSB</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle name="UMR7278" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Sénégal</country><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName><country>France</country><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author></analytic><idno type="DOI">10.1186/s12936-017-1773-4</idno><series><title level="j">Malaria Journal</title><idno type="ISSN">1475-2875</idno><imprint><date type="datePub">2017-03</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>Background: In response to increasing resistance to anti-malarial drugs, Senegal adopted artemisinin-based combination therapy ( ACT) as the first-line treatment for uncomplicated malaria in 2006. However, resistance of Plasmodium falciparum parasites to artemisinin derivatives, characterized by delayed parasite clearance after treatment with ACT or artesunate monotherapy, has recently emerged and rapidly spread in Southeast Asia. After 10 years of stability with rates ranging from 5.6 to 11.8%, the prevalence of parasites with reduced susceptibility in vitro to monodesethylamodiaquine, the active metabolite of an ACT partner drug, increased to 30.6% in 2014 in Dakar. Additionally, after a decrease of the in vitro chloroquine resistance in Dakar in 2009-2011, the prevalence of parasites that showed in vitro chloroquine resistance increased again to approximately 50% in Dakar since 2013. The aim of this study was to follow the evolution of the susceptibility to ACT partners and other anti- malarial drugs in 2015 in Dakar. An in vitro test is the only method currently available to provide an early indication of resistance to ACT partners. Results: Thirty-two P. falciparum isolates collected in 2015 in Dakar were analysed using a standard ex vivo assay based on an HRP2 ELISA. The prevalence of P. falciparum parasites with reduced susceptibility in vitro to monodesethylamodiaquine, chloroquine, mefloquine, doxycycline and quinine was 28.1, 46.9, 45.2, 31.2 and 9.7%, respectively. None of the parasites were resistant to lumefantrine, piperaquine, pyronaridine, dihydroartemisinin and artesunate. These results confirm an increase in the reduced susceptibility to monodesethylamodiaquine observed in 2014 in Dakar and the chloroquine resistance observed in 2013. The in vitro resistance seems to be established in Dakar. Additionally, the prevalence of parasites with reduced susceptibility to doxycycline has increased two-fold compared to 2014. Conclusions: The establishment of a reduced susceptibility to monodesethylamodiaquine as well as chloroquine resistance, and the emergence of a reduced susceptibility to doxycycline are disturbing. The in vitro and in vivo surveillance of anti- malarial drugs must be implemented in Senegal.</p> </div></front></TEI><affiliations><list><country><li>France</li><li>Sénégal</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement><orgName><li>Université d'Aix-Marseille</li></orgName></list><tree><country name="Sénégal"><noRegion><name sortKey="Diawara, Silman" sort="Diawara, Silman" uniqKey="Diawara S" first="Silman" last="Diawara">Silman Diawara</name></noRegion><name sortKey="Amalvict, Remy" sort="Amalvict, Remy" uniqKey="Amalvict R" first="Remy" last="Amalvict">Remy Amalvict</name><name sortKey="Bercion, Raymond" sort="Bercion, Raymond" uniqKey="Bercion R" first="Raymond" last="Bercion">Raymond Bercion</name><name sortKey="Diatta, Bakary" sort="Diatta, Bakary" uniqKey="Diatta B" first="Bakary" last="Diatta">Bakary Diatta</name><name sortKey="Fall, Becaye" sort="Fall, Becaye" uniqKey="Fall B" first="Bécaye" last="Fall">Bécaye Fall</name><name sortKey="Gueye, Mamadou Wague" sort="Gueye, Mamadou Wague" uniqKey="Gueye M" first="Mamadou Wague" last="Gueye">Mamadou Wague Gueye</name><name sortKey="Kounta, Mame Bou" sort="Kounta, Mame Bou" uniqKey="Kounta M" first="Mame Bou" last="Kounta">Mame Bou Kounta</name><name sortKey="Lo, Gora" sort="Lo, Gora" uniqKey="Lo G" first="Gora" last="Lo">Gora Lo</name><name sortKey="Madamet, Marylin" sort="Madamet, Marylin" uniqKey="Madamet M" first="Marylin" last="Madamet">Marylin Madamet</name><name sortKey="Nakoulima, Aminata" sort="Nakoulima, Aminata" uniqKey="Nakoulima A" first="Aminata" last="Nakoulima">Aminata Nakoulima</name><name sortKey="Pradines, Bruno" sort="Pradines, Bruno" uniqKey="Pradines B" first="Bruno" last="Pradines">Bruno Pradines</name><name sortKey="Wade, Khalifa Ababacar" sort="Wade, Khalifa Ababacar" uniqKey="Wade K" first="Khalifa Ababacar" last="Wade">Khalifa Ababacar Wade</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000103 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000103 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= France
|étape= Analysis
|type= RBID
|clé= Hal:hal-01521245
|texte= Confirmation of Plasmodium falciparum in vitro resistance to monodesethylamodiaquine and chloroquine in Dakar, Senegal, in 2015
}}
| This area was generated with Dilib version V0.6.33. |  |